mouse mmp13  (Cusabio)

Journal: Journal of Pharmaceutical Analysis

Article Title: A novel exploration of COL11A1 's role in regulating myeloid-derived suppressor cell activation within the colon cancer microenvironment

doi: 10.1016/j.jpha.2024.101181

Figure Lengend Snippet: Effects of collagen type XI alpha 1 ( COL11A1 ) on growth, proliferation, and migration of cancer-associated fibroblasts (CAFs). (A) COL11A1 , matrix metalloproteinase ( MMP )3, and MMP13 messenger RNA (mRNA) expression in normal fibroblasts (NFs) and CAFs. (B) Protein expression of COL11A1, MMP3, and MMP13 in NFs and CAFs. (C) Enzyme-linked immunosorbent assay (ELISA) detection of MMP3 and MMP13 content in supernatant of bone marrow cells (BMCs) co-cultured with NFs and CAFs. (D) mRNA expression levels of COL11A1 after knockout. (E) Protein expression levels of COL11A1 after knockout. (F) Cell counting kit (CCK)-8 assay to evaluate the growth activity of CAFs after COL11A1 knockout ( COL11A1 KO ). (G) 5-Ethynyl-2′-deoxyuridine (EdU) labeling to measure the proliferation rate of CAFs after COL11A1 KO . (H) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining to assess the apoptosis rate of CAFs after COL11A1 KO . (I) Wound healing experiment to determine the migration ability of CAFs after COL11A1 KO . (J) Expression of relevant proteins in CAFs after COL11A1 KO . ∗∗ P < 0.01; all cell experiments were repeated three times. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; OD: optical density; DAPI: 4′,6-diamidino-2-phenylindole; p-PI3K: phosphorylated-phosphoinositide 3-kinase (PI3K); p-AKT: phosphorylated-protein kinase B (AKT); p-mTOR: phosphorylated mechanistic target of rapamycin (mTOR).

Article Snippet: The levels of matrix metalloproteinase (MMP)3 and MMP13 in the cell supernatant were detected using the mouse MMP3 ELISA kit (D721078, Bioss, Beijing, China) and mouse MMP13 ELISA kit (D721035, Bioss, Beijing, China).

Techniques: Migration, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Knock-Out, Cell Counting, CCK-8 Assay, Activity Assay, Labeling, TUNEL Assay, Staining

Journal: Journal of Pharmaceutical Analysis

Article Title: A novel exploration of COL11A1 's role in regulating myeloid-derived suppressor cell activation within the colon cancer microenvironment

doi: 10.1016/j.jpha.2024.101181

Figure Lengend Snippet: Effects of collagen type XI alpha 1 ( COL11A1 ) in cancer-associated fibroblasts (CAFs) on myeloid-derived suppressor cells (MDSCs) differentiation and activation. (A) Enzyme-linked immunosorbent assay (ELISA) detection of matrix metalloproteinase (MMP)3 and MMP13 content in supernatant of bone marrow cells (BMCs) co-cultured with COL11A1 knockout ( COL11A1 KO ) CAFs. (B) Flow cytometry analysis of arginase 2 ( ARG2 ) and CD11b expression frequency in BMCs co-cultured with COL11A1 KO CAFs. (C) ARG2 and CD11b messenger RNA (mRNA) expression levels in BMCs co-cultured with COL11A1 KO CAFs. (D) ARG2 and CD11b protein expression levels in BMCs co-cultured with COL11A1 KO CAFs. (E) Flow cytometry analysis of interferon gamma (IFN-γ) secretion in CD8 + T cells. (F) Flow cytometry analysis of granzyme B (GZMB) secretion in CD8 + T cells. (G) Flow cytometry analysis of tumor necrosis factor alpha (TNF-α) secretion in CD8 + T cells. (H) Flow cytometry analysis of apoptosis in CD8 + T cells. (I) Flow cytometry analysis of proliferation in CD8 + T cells. ∗∗ P < 0.01; all cell experiments were repeated three times. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; FITC: fluorescein isothiocyanate; CFSE: carboxyfluorescein succinimidyl ester.

Article Snippet: The levels of matrix metalloproteinase (MMP)3 and MMP13 in the cell supernatant were detected using the mouse MMP3 ELISA kit (D721078, Bioss, Beijing, China) and mouse MMP13 ELISA kit (D721035, Bioss, Beijing, China).

Techniques: Derivative Assay, Activation Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Knock-Out, Flow Cytometry, Expressing

Journal: Journal of Pharmaceutical Analysis

Article Title: A novel exploration of COL11A1 's role in regulating myeloid-derived suppressor cell activation within the colon cancer microenvironment

doi: 10.1016/j.jpha.2024.101181

Figure Lengend Snippet: Impact of collagen type XI alpha 1 ( COL11A1 ) activation through myeloid-derived suppressor cells (MDSCs) on colon cancer (CC) tumor growth and proliferation. (A) Growth of CC orthotopic xenografts in mice after COL11A1 knockout. (B) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining to evaluate cell apoptosis in CC orthotopic xenografts in mice after COL11A1 knockout. (C) Ki67 staining to assess cell proliferation in CC orthotopic xenografts in mice after COL11A1 knockout. (D) Immunohistochemical detection of COL11A1 and arginase 2 ( ARG2 ) expression in CC orthotopic xenografts in mice after COL11A1 knockout. (E) Immunohistochemical detection of matrix metalloproteinase ( MMP )3 and MMP13 expression in CC orthotopic xenografts in mice after COL11A1 knockout. (F) Flow cytometry analysis of MDSC differentiation and activation in CC orthotopic xenografts in mice after COL11A1 knockout ( n = 6 per group). ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: The levels of matrix metalloproteinase (MMP)3 and MMP13 in the cell supernatant were detected using the mouse MMP3 ELISA kit (D721078, Bioss, Beijing, China) and mouse MMP13 ELISA kit (D721035, Bioss, Beijing, China).

Techniques: Activation Assay, Derivative Assay, Knock-Out, TUNEL Assay, Staining, Immunohistochemical staining, Expressing, Flow Cytometry

Journal: Journal of Pharmaceutical Analysis

Article Title: A novel exploration of COL11A1 's role in regulating myeloid-derived suppressor cell activation within the colon cancer microenvironment

doi: 10.1016/j.jpha.2024.101181

Figure Lengend Snippet: Collagen type XI alpha 1 ( COL11A1 ) induces the paracrine secretion of matrix metalloproteinase (MMP)3 and MMP13 in cancer-associated fibroblasts (CAFs), promoting colon cancer (CC) development and immune escape (Created by using www.biorender.com ).

Article Snippet: The levels of matrix metalloproteinase (MMP)3 and MMP13 in the cell supernatant were detected using the mouse MMP3 ELISA kit (D721078, Bioss, Beijing, China) and mouse MMP13 ELISA kit (D721035, Bioss, Beijing, China).

Techniques:

Journal: Frontiers in Physiology

Article Title: TGF-β signaling in the cranial neural crest affects late-stage mandibular bone resorption and length

doi: 10.3389/fphys.2024.1435594

Figure Lengend Snippet: TaqMan primers. TaqMan primers for Mmp13 , Mmp9 , Rankl , Opg , Rank , and M-csf are listed along with their amplicon lengths.

Article Snippet: Primers for mouse Mmp13, Mmp9, Rank, Rankl, Opg , and M-csf are commercially available from Thermo Fisher Scientific (see for primer sequences).

Techniques: Amplification, TaqMan Assay

Journal: The Journal of Poultry Science

Article Title: Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens

doi: 10.2141/jpsa.2024018

Figure Lengend Snippet: List of partially representative differentially expressed genes (DEGs).

Article Snippet: Mouse monoclonal antibodies against MMP13 (Thermo, New York, USA, 1:1000) were added and incubated overnight, followed by the addition of Cy3-labeled fluorescent secondary antibody (Thermo, New York, USA, 1:1000) for 1 h. 4′,6-diamidino-2-phenylindole was added to stain nuclei and images were collected using a fluorescence microscope.

Techniques: Activity Assay, Binding Assay

Journal: The Journal of Poultry Science

Article Title: Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens

doi: 10.2141/jpsa.2024018

Figure Lengend Snippet: Validation of matrix metalloproteinase 13 (MMP13) by immunofluorescence (IF) (A) and western blotting (WB) (B). BCs, broody chickens; EHs, egg-laying hens; OC, oocyte; GC, granular cell; TC, theca cell.

Article Snippet: Mouse monoclonal antibodies against MMP13 (Thermo, New York, USA, 1:1000) were added and incubated overnight, followed by the addition of Cy3-labeled fluorescent secondary antibody (Thermo, New York, USA, 1:1000) for 1 h. 4′,6-diamidino-2-phenylindole was added to stain nuclei and images were collected using a fluorescence microscope.

Techniques: Biomarker Discovery, Immunofluorescence, Western Blot

Journal: The Journal of Poultry Science

Article Title: Ovary Transcriptome Profiling in Broody and Egg-laying Chahua Chickens

doi: 10.2141/jpsa.2024018

Figure Lengend Snippet: Summary of possible mechanisms that cause development of follicles and ovulation in the ovary. Follicle-stimulating hormone (FSH), prolactin (PRL), and estrogen (E 2 ) may participate in the mechanisms orchestrating extracellular matrix turnover mediated by matrix metalloproteinase 13 (MMP13) in the Chahua hen ovary, regulating ovarian development, ovulation, and affecting chicken broodiness. ECM, extracellular matrix.

Article Snippet: Mouse monoclonal antibodies against MMP13 (Thermo, New York, USA, 1:1000) were added and incubated overnight, followed by the addition of Cy3-labeled fluorescent secondary antibody (Thermo, New York, USA, 1:1000) for 1 h. 4′,6-diamidino-2-phenylindole was added to stain nuclei and images were collected using a fluorescence microscope.

Techniques: